Decontaminate PCR work areas  Ready-to-use Cost effective

Non-carcinogenic Heat resistant

DNA-OFF™ is a non-alkaline cleansing solution that is highly active against DNA contamination. This solution contains a surfactant plus an agent that destroys DNA. DNA-OFF™ is intended for use at PCR workstations, including countertops, apparatus and pipettors. Presence of contaminant DNA at a PCR work area can result in DNA amplification artifacts. DNA-OFF™ has been demonstrated to effectively remove DNA contamination (30 pg p53 DNA template) from a countertop as evidenced by the inability to generate a PCR product after cleaning (Figure 1).

The DNA-OFF™ decontamination solution is stable and heat resistant. In addition, DNA-OFF™ does not contain carcinogenic compounds.

DNA-OFF™ is ready-to-use for eliminating DNA from all surfaces. The decontamination instructions are easy to follow. By simply applying DNA-OFF™ and rinsing, DNA is completely removed leaving your work area free of contaminant DNA. It is available in a spray bottle and also an economical refill size.

Figure 1

Effectiveness of DNA-OFF at removal of DNA contamination. Increasing amounts of p53 template DNA were dried onto the bottom of thin wall PCR tubes. DNA-OFF™ (100 µl) was added to the tubes as indicated and 100 µl ddH₂O was added to the control tube. Each tube was vortexed for 20 seconds followed by two 100 µl washes with ddH₂O. PCR reaction mix containing 15 pg IL-1b DNA template and both IL-1b and p53 were added to each tube and run for 35 cycles. PCR products were resolved on a 1.2% agarose gel (1X TAE) and visualized by EtBr staining.