Researcher: I am working with a mutant strain of Schizosaccharomyces pombe that will not reach normal cell density with overnight culture (recommended in the kit). How will I determine how much of this culture I should use with your DNA-Pure™ Yeast Genomic Kit?

TechTALKer:This is a typical concern. You can either grow the cells longer or use a larger volume of the cell culture to optimize your DNA yields. The following table gives yields obtained as a function of OD at 595 nm. You can use the following table as a guide to determine what your yield should be per ml of cell culture at various stages of growth and hence the volume of cell culture to use.

For example, if your culture only has 1x106 cells/ml after overnight growth in order to obtain maximum yield you should use 7.5 to 15 ml culture rather than the 1.5 ml recommended in the kit protocol. Increasing the volume of cell culture in the sample does not require increasing the volume of kit buffers needed for further processing. As you can see, when the cells enter the stationary phase DNA yields drop, so it is best to avoid such samples.

Related Product Pages

• DNA-Pure™ Yeast Genomic Kit